Please contact Zhanyuan Jon Zhang via e-mail (firstname.lastname@example.org), by phone(573-882-6922), or meet in our transformation lab to discuss your service request.
If you are located outside the state of Missouri, please contact USDA-APHIS as soon as possible, to request permission for cross-state movement of transgenic materials. International users wonít need cross-state movement permit.
You will subclone your gene expression cassette into binary vector pZY101, pZY102, pMU103, pMU104,
pFGC5941, pEarleyGate series (containing bar cassette), or other vectors published or verified by PTCF. Both pFGC5941 and pEarleyGate series can be purchased from TAIR (The Arabidopsis Information Resource). Users need to contact us to discuss the constructs of choices and required cloning strategy before vector construction.
For off-campus users, we will mobilize the vector into Agrobacterium cells and confirm its integrity within the cells at a charge of $150/vector confirmation. Please provide the restriction map of your construct for our confirmation work.
Users are encouraged to request empty vector control events. This will not only benefit the userís publication requirement but also allow evaluating if userís genes of interest have any negative impact on plant transformation in our side-by-side service experiments.
Standard time for service deliverables can be found under the ďServiceĒ for each crop species.
When users confirm the vector integrity within the Agrobacterium, PCR-based confirmation will no longer be considered as a valid proof, and users are highly recommended to show digestion of the final transformation vector rescued from bacterial cells carrying the construct.