Service Request Procedure

  1. Please contact Zhanyuan Jon Zhang via e-mail (zhangzh@missouri.edu),
    by phone(573-882-6922), or meet in our transformation lab to discuss your service request.

  2. If you are located outside the state of Missouri, please contact USDA-APHIS as soon as possible, to request permission for cross-state movement of transgenic materials. International users won’t need cross-state movement permit.

  3. You will subclone your gene expression cassette into binary vector pZY101, pZY102, pMU103
    pFGC5941, pEarleyGate series (containing bar cassette), or other vectors published or verified by PTCF. Off-campus users should contact Addgene to get pZY101, pZY102, or pMU103 by typing in the plasmid names in the “Search” area. Both pFGC5941 and pEarleyGate series can be purchased from TAIR (The Arabidopsis Information Resource). Users should contact us to discuss the constructs of choices before vector construction because we use only certain constructs to transform some crop species.

  4. For off-campus users, we will mobilize the vector into Agrobacterium cells and confirm its integrity within the cells at a charge of $150/vector confirmation. Please provide the restriction map of your construct for our confirmation work.

Service Note
  1. Users are encouraged to request empty vector control events. This will not only benefit the user’s publication requirement but also allow evaluating if user’s genes of interest have any negative impact on plant transformation in our side-by-side service experiments.
  2. Standard time for service deliverables can be found under the “Service” for each crop species.
  3. When users confirm the vector integrity within the Agrobacterium, PCR-based confirmation will no longer be considered as a valid proof, and users are highly recommended to show digestion of the final transformation vector rescued from bacterial cells carrying the construct. This new requirement will benefit users as well as our operation because of more thorough examination of those steps before the transformation experiments start.

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