Service Request Procedure
- Please contact Zhanyuan Jon Zhang via e-mail (firstname.lastname@example.org), by phone (573-882-6922), or meet in our transformation lab to discuss your service request.
- After the initial contact, we will send you an invoice for payment. After receiving the payment, we will start transformation experiments. If you are located outside the state of Missouri, please contact USDA-APHIS as soon as possible, to request permission for cross-state movement of transgenic materials.
- You will subclone your gene expression cassette into binary vector pZY101, pZY101.2, pFGC5941(-), pFGC5941, pEarleyGate series (containing bar cassette), or other vectors published or verified by PTCF. Off-campus users will sign a Material Transfer Agreement sheet to use any of pZY101, pZY101.2 or pFGC5941(-). Both pFGC5941 and pEarleyGate series can be purchased from TAIR (The Arabidopsis Information Resource) without going through MTA process. We will mobilize the vector into Agrobacterium cells and confirm its integrity within the cells at a charge of $150/vector confirmation. Please provide the restriction map of your construct for our confirmation work.
Below are what we would like to communicate with our users so the requirements and expectations will be clear before the service experiments start.
- Users are encouraged to request empty vector control events. This will not only benefit the user’s publication requirement but also allow evaluating if user’s genes of interest have any negative impact on plant transformation in our side-by-side service experiments.
- Standard time for service deliverables can be found under the “Service” for each crop species.
- When users confirm the vector integrity within the Agrobacterium, PCR-based confirmation will no longer be considered as a valid proof, and users are highly recommended to show digestion of the final transformation vector rescued from bacterial cells carrying the construct. This new requirement will benefit users as well as our operation because of more thorough examination of those steps before the transformation experiments start.